Which enzyme is primarily involved in glycolysis?

Hexokinase is the enzyme primarily involved in glycolysis because it catalyzes the first step of this metabolic pathway, which is the phosphorylation of glucose to form glucose-6-phosphate. This reaction is crucial as it effectively traps glucose within the cell and prepares it for further breakdown in the glycolytic pathway. Hexokinase plays a vital role in regulating glucose metabolism and is significant in energy production.

The other enzymes listed have distinct functions unrelated to glycolysis. Amylase is responsible for the breakdown of starches into simple sugars; lipase is involved in the digestion of lipids (fats); and protease breaks down proteins into amino acids. These enzymes operate in different metabolic pathways and do not participate in the glycolytic process.