Which method is NOT typically used for hemoglobin measurement?

Centrifugation is not typically used for hemoglobin measurement because it is primarily a physical separation technique applied to separate components in a liquid sample based on their density. While centrifugation is important in the processing of blood samples (such as separating plasma from cells), it does not measure hemoglobin levels directly.

In contrast, manual methods, automated methods, and electrophoresis are all well-established techniques for quantifying hemoglobin. Manual methods include colorimetric assays where hemoglobin is reacted with specific reagents to produce a measurable color change. Automated methods utilize sophisticated equipment that can rapidly measure hemoglobin concentration along with other blood parameters. Electrophoresis is used to separate different types of hemoglobin based on their charge and is particularly useful for identifying hemoglobin variants, such as in the diagnosis of hemoglobinopathies.

Thus, the best choice for what is not typically used for measuring hemoglobin levels is centrifugation, which serves a different purpose in lab procedures.